# Maloof Lab Databases [Arabidopsis seeds (internal only ask Kazu to access)](https://docs.google.com/spreadsheets/d/1TYf6AcCbsYUQVU4Nx-dy0-ZmJc2AHVkYrssT4QPsmrc/edit#gid=637084952) [Tomato seeds (internal only ask Kazu to access)](https://docs.google.com/a/ucdavis.edu/spreadsheets/d/14wwx79O5uw2hurfFEjw1r8XclUuaZAdDPgJodO0XFeA/edit?usp=sharing) [Glycerol stock of plasmids and strains (internal only ask Kazu to access)](https://docs.google.com/a/ucdavis.edu/spreadsheets/d/1gZo5isGAMYD6Kog8IYasVlfnPReGb5wOX7Pm1YgSJmU/edit?usp=sharing) Maloof lab oligo (internal only ask Kazu to access)(Find "oligos" under "Maloof Lab Common" in Projects at benchling.com) [Maloof lab order history (internal only ask Jiadong to access)](https://docs.google.com/spreadsheets/d/1It3uvBEt8L5mNA6Ed1W8KPCCItzAyaQF5fiB9l-Ojoo/edit#gid=237921429) [Maloof lab ordering form (internal only ask Jiadong to access)](https://docs.google.com/forms/d/1PZrW1vBr4rZL3m9RABKFJDDMKVRVGnqzaBzdMHmcv8s/edit) # Tools plasmid construction (Use benchling.com) Maloof lab oligo search (Use benchling.com) [Gene structure and oligo position visualization](https://github.com/MaloofLab/primer_position) [[Assembling Sanger DNA sequence data]] # Protocols [[agroinfiltration]] [SLiCE (Seamless L i gation Cloning Extract)](http://malooflab.phytonetworks.org/wiki/common_lab_and_mol_bio_protocols/slice/) [Homemade taq system](http://malooflab.phytonetworks.org/wiki/common_lab_and_mol_bio_protocols/homemade_taq_system/) [[Tomato Seed Collection]] plant DNA extraction1 For 1.5 ml tube or 96 well plates (Gasser Lab protocol via Rod Kumimoto) [attachment:20] [[plant DNA extraction2]] For 96 well plates(CTAB Method) [[plant DNA precipitation]] For 96 well plates (With glycogen) [[covaris_dna_shearing]] [[Ultra-competent E coli]] (Inoue Method) [[Cell transformation]] DH5α chemically-competent [[Agrobacterium transformation]] Agrobacterium (Freeze-Thaw method) [[Cleaning ball bearings]] [[Arabidopsis seed sterilization]] [[Miniprep using Promega reagents]] but not the columns [[Miniprep solutions recipes]] [[MS Plate Recipes]] for plant growth [[ExoSAP PCR cleanup]] for sequencing [[Seed Storage]] [Eleven Golden Rules of qRTPCR](http://www.plantcell.org/cgi/reprint/20/7/1736) Uvardi et al. [[Trizol Extraction]] [[Tomato Seed Collection]] TMV treatment and sterilization # Stocks [[Antibiotics]] [[Electrophoresis Buffers]] [[Loading Dye and 1kb Ladder]] [[PCR Supplies]] Taq, Buffer, and MgCl2 # [[Undergrad Instructions]] # Scripts, instructions and miscellaneous [[Epson scanner]] [[LI1800 light meter]] Black Comet light meter [attachment:23] [[Sequence analysis with Benchling]] [[Analyzing luminiscence]] over time with ImageJ [[Hypocotyl measurement]] with ImageJ [[Maloof and Harmer Primer Database Search]] (Only internal users) [[Patricias Motif search]] (Only internal users) [[Maloof Gene Expression Plot Tool]] in different tissues for M82 vs PEN (Only internal users) [[Maloof Gene Expression Plot Tool]] for M82, PEN, HAB & PIM (Only internal users) [[Printing Laser Tough Tag Settings]] [[Printing barcodes]] [[PDA measurement system]] [[Measuring time-lapse hypocotyl movies]] with ImageJ [[Earth champion instructions]]