This protocol will make 1 liter of 50x solution. Adjust to your needs if necessary.
Add 242 g of Tris base
57.1 ml of glacial acetic acid
100 ml of 0.5M EDTA (pH 8.0)
Add water to 1 liter
This protocol will make 2 liter of 50x solution. Adjust to your needs if necessary.
Add 40 g of NaOH into about 1.8 liters of H2O
pH to 8.0 with Boric Acid (about 180 g)
Add water to 2 liters