For use with econospin miniprep protocol or miniprep without columns protocol ([Wizard Plus SV mini-prep system](https://www.promega.com/~/media/Files/Resources/Protocols/Technical%20Bulletins/0/Wizard%20Plus%20SV%20Minipreps%20DNA%20Purification%20System%20Protocol.pdf))

## Buffer 1 (Cell resuspension buffer)  
50mM Tris  
10mM EDTA  
100 ug/mL RNase A  
For 100 mL  
5 mL 1M Tris pH8  
2 mL 0.5M EDTA  
1 mL 10mg/mL RNase A  
92 mL water  

## Buffer 2 (Cell lysis solution)  
0.2M NaOH  
1% SDS  
For 100 mL  
20 mL 1M NaOH  
5 mL 20% SDS  
75 mL water  

## Buffer 3 (Neutralization Solution)  
4M guanidine hydrochloride  
0.5M sodium acetate  
For 100 mL  
2.3g guanidine hydrochloride  
4.9g potassium acetate  
pH to 4.2 with glacial acetic acid  
bring volume to 100 mL with water  

## Wash buffer  
60mM potassium acetate  
8.3mM Tris-HCl (pH 7.5)  
40μM EDTA (pH 8)  
60% ethanol (For 100mL)  
1.2 mL 5M potassium acetate  
0.83 mL 1M Tris (pH 7.5)  
8 μL 0.5M EDTA  
60 mL 100% ethanol  
38mL water  

ADD 170 mL 95% ethanol to the wash buffer before first use!!