• We have much tomato seed storage in the lab, but if you want to collect some new fresh tomato seeds by yourself, you might need this protocol.
  • Cut the fruits along the diameter
  • Collect the seed in fruit locule without placenta, put them in a plastic box (each box for one plant labeled with paper tab)
  • Prepare 1% digestive enzyme for seed processing (the original enzyme solution is stored in the cold room of LSA 2214)
  • Add 10 x volume of 1% enzyme solution to the plastic box with tomato seed tissue
  • Keep them around 2 hours
  • Wash the seed under the faucet, get rid of other fruit tissue
  • Dry them on the toilet paper
  • Collect them in the seed envelops and label with sample name and collecting date,etc.