We grow plants in the Controlled Environment Facility. Please register as a CEF user before using this facility.
Space in the CEF is coordinated by Kazu
CEF501(a big walk in chamber equipped with simulated sun (right side) or shade (left side) condition; calendar is under construction)
CEF64 (a small growth chamber)
CEF65 (a small growth chamber)
CEF503 (Small right hand walk in chamber in CEFB)
CEF504 (Small walk-in chamber on Left hand side in CEF B. Please specify which Shelf # you will be using.)
For seedling experiments we have two "custom" LED chambers with Quantum Devices SnapLites. These are in the basement of Briggs and are called Maloof Left LEDs and Maloof Right LEDs on Google Calendar used for sign up.
We also have an Andor CCD camera for luciferase imaging. This is in a Percival chamber with red, far-red, and blue LEDs. This is known as Kirkwood on the Google Calendar sign up.
For permission to sign up for these chambers please contact Kazu
Maloof Left ICAL feed:
Maloof Right ICAL feed:
Kirkwood ICAL feed:
light condition (LED chambers): R (34 µE) + B (7 µE) and FR adjusted (R/FR = 1.3 for simulated sun and 0.5 for simulated shade).
Switch right and left chambers due to possible temperature differences. Eg. Replicate 1 (left chamber (R/FR=1.3) and right chamber (R/FR=0.5), Replicate 2 (left chamber (R/FR=0.5) and right chamber (R/fR=1.3).
Use squire plates without grids for scanning plates and place plates at vertical position in growth chambers (use 1ml blue tips and tip boxes as custom plate holders).
After four days stratification in a tin box, let seeds germinate under simulated sun chamber for three days and score non-germinated seeds on the third day (eliminate slow germinators from data analysis).
Split plates into simulated sun and shade and grow plants for four days and scan plates.
Sprinkle ethanol sterilized seeds on plates 5 mm apart (in two or three rows). You can use marked grid square plate as position template underneath of your plates.
Randomize genotypes position within plate to avoid position effects.
We rent greenhouse space in the Orchard Park Greenhouse Facility. Before using greenhouse, take greenhouse training (contact Ron Lane, firstname.lastname@example.org). And then contact Plant Biology greenhouse manager (Ian Baker email@example.com).
Before you begin to grow you plants in the greenhouse, please first finish greenhouse Safety Trainning