This protocol will make 4 liters. Adjust to your needs if necessary. Put 3500 ml Milli-Q H2O in a beaker Weight 17.6 g of MSMO (in the 4°C freezer, 4.4 per liter) and add it to the water (agitating) Add 30 g/l (120 g for 4 liters) Sucrose Adjust pH to 5.7 (adding KOH if it’s lower) Fill with Milli-Q water to exactly 4 liters Put 7 g of Agar (per bottle) in 4 clean glass bottles and add 1 liter of the mix to each bottle Autoclave for 35 min Let cool, add antibiotic to your convenience and pour into plates Big round plates take about 50 ml of media Square plates take about 25 ml of media