MS plates for plant growth (buffered, no sucrose)
- Final concentration of this medium is 1/2 MSMO, 5mM MES (pH5.8), 0.8% (or 0.7%) agar.
- Put 700 ml Milli-Q H2O in a beaker
- Weight 2.2 g of MSMO (in the 4°C freezer) and add it to the water (agitating)
- Add 1.0g of MES (2-(N-morpholino)ethanesulfonic acid)
- Adjust pH to 5.8 (adding KOH)
- Fill with Milli-Q water to exactly 1 liters
- Put 8 g (for vertical plate) or 7 g (for horizontal plate) of Agar in 1 clean glass bottle
- Autoclave for 30 min
- Let cool, add antibiotic to your convenience and pour into plates.
- Big round plates take about 50 ml of media.
- Square plates take 25 ml of media (for horizontal plate) or 40 ml of media (for vertical plate).
- created by Kazu (Oct 25, 2012)