MS plates for plant growth (buffered, no sucrose) ------------------ * Final concentration of this medium is 1/2 MSMO, 5mM MES (pH5.8), 0.8% (or 0.7%) agar. * Put 700 ml Milli-Q H2O in a beaker * Weight 2.2 g of MSMO (in the 4°C freezer) and add it to the water (agitating) * Add 1.0g of MES (2-(N-morpholino)ethanesulfonic acid) * Adjust pH to 5.8 (adding KOH) * Fill with Milli-Q water to exactly 1 liters * Put 8 g (for vertical plate) or 7 g (for horizontal plate) of Agar in 1 clean glass bottle * Autoclave for 30 min * Let cool, add antibiotic to your convenience and pour into plates. * Big round plates take about 50 ml of media. * Square plates take 25 ml of media (for horizontal plate) or 40 ml of media (for vertical plate). * created by Kazu (Oct 25, 2012)